Nhanganyaya yeSmall Peptide Trace Mineral Chelates
Chikamu 1 Nhoroondo yeZvimwe Zvicherwa Zvinowedzera
Inogona kukamurwa kuita zvizvarwa zvina zvichienderana nekugadzirwa kwezvinowedzera zvicherwa:
Chizvarwa chekutanga: Munyu wezvicherwa zvisingawanikwe, zvakaita se copper sulfate, ferrous sulfate, zinc oxide, nezvimwewo; Chizvarwa chechipiri: Munyu we organic acid wezvicherwa zvisingawanikwe, zvakaita se ferrous lactate, ferrous fumarate, copper citrate, nezvimwewo; Chizvarwa chechitatu: Amino acid chelate feed grade yezvicherwa zvisingawanikwe, zvakaita se zinc methionine, iron glycine uye zinc glycine; Chizvarwa chechina: Munyu weprotein uye munyu we peptide chelating wezvicherwa zvisingawanikwe, zvakaita se protein copper, protein iron, protein zinc, protein manganese, small peptide copper, small peptide iron, small peptide zinc, small peptide manganese, nezvimwewo.
Chizvarwa chekutanga chine zvicherwa zvisingasanganisike, uye chizvarwa chechipiri kusvika chechina chine zvicherwa zvisingasanganisike.
Chikamu 2 Sei Uchifanira Kusarudza MaChelates Madiki ePeptide
Ma peptide chelates madiki ane simba rinotevera:
1. Kana mapeptide madiki akabatana nemaion esimbi, anenge akapfuma muzvimiro uye akaoma kuwanda;
2. Haikwikwidzane nemigero yeamino acid, ine nzvimbo dzakawanda dzekunyudzwa uye inokurumidza kunyudzwa;
3. Kushandiswa kwesimba shoma; 4. Kuwanda kwemari inochengetwa, mwero wekushandiswa kwepamusoro uye kushanda kwemhuka kwakawedzera zvikuru;
5. Inorwisa mabhakitiriya uye inorwisa mabhakitiriya;
6. Kudzora dziviriro yemuviri.
Ongororo dzakawanda dzakaratidza kuti hunhu kana mhedzisiro ye small peptide chelates inoita kuti ive netarisiro yakakura yekushandisa uye mukana wekukura, saka kambani yedu pakupedzisira yakasarudza kutora small peptide chelates senzvimbo yekutsvagisa nekugadzira zvigadzirwa zve organic trace mineral zvekambani.
Chikamu 3 Kushanda zvakanaka kwe peptide chelates diki
1. Hukama huri pakati pemapeptide, maamino acids nemapuroteni
Huremu hwemamorekuru eprotein hunopfuura chiuru;
Huremu hwemamorekuru epeptide i150 ~ 10000;
Mapeptide madiki, anonziwo mapeptide madiki emamorekuru, ane maamino acid maviri kusvika mana;
Avhareji yehuremu hwemaamino acids inenge 150.
2. Kubatanidza mapoka emaamino acids nemapeptides ane chelated nesimbi
(1) Mapoka anobatanidza maamino acids
Mapoka ekubatanidza mumaamino acids:
Mapoka eAmino neCarboxyl ari paA-carbon;
Mapoka emacheni emamwe maa-amino acids, akadai se sulfhydryl group ye cysteine, phenolic group ye tyrosine uye imidazole group ye histidine.
(2) Kuronga mapoka mumapeptide madiki
Mapeptide madiki ane mapoka akawanda anobatanidza zvinhu kupfuura maamino acids. Kana akachera nemaion esimbi, zviri nyore kuchera, uye anogona kugadzira multidentate chelation, izvo zvinoita kuti chelate igadzikane.
3. Kushanda zvakanaka kwechigadzirwa che peptide chelate chidiki
Hwaro hwepfungwa dze small peptide inokurudzira kunyudzwa kwemaminerari
Hunhu hwekunyudzwa kwemapeptide madiki ndiwo hwaro hwedzidziso yekusimudzira kunyudzwa kwezvinhu zvidiki. Zvinoenderana nedzidziso yechinyakare yeprotein metabolism, zvinodiwa nemhuka kuti dzive nemapuroteni ndizvo zvavanoda pamaamino acid akasiyana-siyana. Zvisinei, mumakore achangopfuura, zvidzidzo zvakaratidza kuti chiyero chekushandiswa kwemaamino acids muzvikafu zvinobva kunzvimbo dzakasiyana chakasiyana, uye kana mhuka dzikadyiswa chikafu chine homozygous kana chikafu chine protein yakaderera amino acid balanced, kugona kwakanakisa kwekugadzira hakugone kuwanikwa (Baker, 1977; Pinchasov et al., 1990) [2,3]. Nokudaro, vamwe vadzidzi vakapa maonero ekuti mhuka dzine kugona kwakakosha kwekunyudzwa kwemapuroteni asina kunyudzwa pachawo kana mapeptide ane hukama. Agar (1953) [4] akatanga kuona kuti nzira yemudumbu inogona kunyudzwa zvizere uye kutakura diglycidyl. Kubva ipapo, vaongorori vakapa nharo inogutsa yekuti mapeptide madiki anogona kunyudzwa zvizere, vachisimbisa kuti glycylglycine isina kunyudzwa inotakurwa uye inonyudzwa; Nhamba huru yemapeptide madiki anogona kunyudzwa zvakananga musystemic circulation muchimiro chemapeptide. Hara et al. (1984)[5] vakataurawo kuti zvigadzirwa zveprotein zvinopinda mudumbu remudumbu zvinonyanya kuva mapeptide madiki pane maamino acids (FAA). Mapeptide madiki anogona kupfuura nemumasero emudumbu zvizere uye kupinda muropa rose (Le Guowei, 1996)[6].
Kufambira Mberi Kwekutsvaga kwePeptide Diki Inokurudzira Kutorwa Kwezvicherwa Zvishoma, Qiao Wei, et al.
Ma peptide chelates madiki anotakurwa uye anonyudzwa muchimiro chema peptide madiki
Zvichienderana nekushandiswa kwemakemikari emushonga uye kutakura uye hunhu hwemapeptide madiki, maminerari madiki anosanganiswa nemapeptide madiki sezvo main ligands anogona kutakurwa ese, izvo zvinobatsira zvikuru pakuvandudza simba remaminerari madiki. (Qiao Wei, et al)
Kushanda kweChelates DzemaPeptide Madiki
1. Kana mapeptide madiki akabatana nemaion esimbi, anenge akapfuma muzvimiro uye akaoma kuwanda;
2. Haikwikwidzane nemigero yeamino acid, ine nzvimbo dzakawanda dzekunyudzwa uye inokurumidza kunyudzwa;
3. Kushandiswa kwesimba shoma;
4. Mari yakawanda inochengetwa, mwero wekushandiswa kwepamusoro uye mashandiro ekugadzira mhuka akawedzera zvikuru;
5. Inorwisa mabhakitiriya uye inorwisa mabhakitiriya; 6. Kudzora masoja emuviri.
4. Kunzwisisa zvakawanda nezve peptides
Ndeupi wevaviri vanoshandisa peptide anowana pundutso yakawanda?
- Peptide inosunga
- Phosphopeptide
- Zvishandiso zvine hukama
- Peptide inorwisa utachiona
- Peptide yemuviri inorwisa mabhakitiriya
- Neuropeptide
- Hormone peptide
- Peptide inorwisa okisijeni
- Mapeptides ane hutano
- Mapeptide ezvinonhuwira
(1) Kupatsanurwa kwemapeptide
(2) Mhedzisiro yemuviri yemapeptides
- 1. Gadzirisa kuenzana kwemvura ne electrolyte mumuviri;
- 2. Kugadzira masoja ekudzivirira chirwere anorwisa mabhakitiriya nehutachiona kuti immune system ishande zvakanaka;
- 3. Kukurudzira kupora kweronda; Kugadzirisa nekukurumidza kukuvara kwenyama ye epithelial.
- 4. Kugadzira ma enzyme mumuviri kunobatsira kushandura chikafu kuita simba;
- 5. Kugadzirisa masero, kunatsiridza kushandiswa kwemasero, kudzivirira kuora kwemasero, uye kuita basa rekudzivirira kenza;
- 6. Kukurudzira kugadzirwa nekugadziriswa kwemapuroteni nema enzymes;
- 7. Mutumwa wemakemikari akakosha ekutumira ruzivo pakati pemasero nenhengo;
- 8. Kudzivirira zvirwere zvemwoyo netsinga dzeropa;
- 9. Kudzora mashandiro e endocrine netsinga.
- 10. Kuvandudza kugaya chikafu uye kurapa zvirwere zvemudumbu zvisingaperi;
- 11. Kuvandudza chirwere cheshuga, rheumatism, rheumatoid nezvimwe zvirwere.
- 12. Kurwisa utachiona, kudzivirira kukwegura, kubvisa ma free radicals akawanda mumuviri.
- 13. Kusimudzira kushanda kweropa, kurapa anemia, kudzivirira kuungana kwemaplatelet, izvo zvinogona kuvandudza kugona kwemasero matsvuku eropa kutakura okisijeni.
- 14. Kurwisa zvakananga mavhairasi eDNA uye kurwisa mabhakitiriya ehutachiona.
5. Basa rekudya kaviri rema peptide chelates madiki
Kamudiki kepeptide chelate kanopinda musero rose mumuviri wemhuka, uyewobva watyora chisungo che chelation otomatikimusero uye inoora kuita ma peptide nema ions esimbi, ayo anoshandiswa nemhuka inoita mabasa maviri ekudyakunyanya iyobasa rekushanda kwepeptide.
Basa re peptide diki
- 1. Kurudzira kugadzirwa kweprotein munyama dzemhuka, kuderedza apoptosis, uye kukurudzira kukura kwemhuka
- 2. Kuvandudza mamiriro emiti yemudumbu uye kukurudzira hutano hwemudumbu
- 3. Ipai carbon skeleton uye wedzerai basa rema enzymes ekugaya chikafu akadai se intestinal amylase ne protease
- 4. Ane mhedzisiro yekushushikana inorwisa oxidative
- 5. Ane hunhu hwekudzivirira kuzvimba
- 6.……
6. Mabhenefiti ema peptide chelates madiki pane ma amino acid chelates
| Amino acid chelated trace minerals | Zvicherwa zvidiki zve peptide chelated trace | |
| Mutengo wezvinhu zvisina kugadzirwa | Zvinhu zvemaamino acid imwe chete zvinodhura | Zvinhu zvakagadzirwa nekeratin muChina zvakawanda. Vhudzi, mahwanda nenyanga zviri mukuchengeta mhuka uye mapuroteni, mvura yakasviba nematehwe zviri muindasitiri yemakemikari zvinhu zvemapuroteni zvemhando yepamusoro uye zvakachipa. |
| Mhedzisiro yekutora | Mapoka eAmino neCarboxyl anobatanidzwa panguva imwe chete mukucheneswa kwemaamino acids nezvinhu zvesimbi, zvichigadzira chimiro chebicyclic endocannabinoid chakafanana neche dipeptides, pasina mapoka ecarboxyl akasununguka aripo, ayo anogona kungotorwa kuburikidza neoligopeptide system. (Su Chunyang et al., 2002) | Kana mapeptide madiki achitora chikamu mu chelation, chimiro che chelation chering imwe chete chinowanzo umbwa ne terminal amino group ne adjoining peptide bond oxygen, uye chelate inochengetedza boka re carboxyl rakasununguka, iro rinogona kunyudzwa kuburikidza ne dipeptide system, rine simba guru rekunyudzwa kupfuura oligopeptide system. |
| Kugadzikana | Maion esimbi ane rimwe kana kupfuura mhete dzine nhengo shanu kana nhanhatu dzemapoka emaamino, mapoka ecarboxyl, mapoka emidazole, mapoka ephenol, uye mapoka esulfhydryl. | Pamusoro pemapoka mashanu aripo ekubatana kwemaamino acids, mapoka ecarbonyl nemaimino mumapeptides madiki anogonawo kubatanidzwa mukubatana, zvichiita kuti ma peptide chelates madiki ave akasimba kupfuura ma amino acid chelates. (Yang Pin et al., 2002) |
7. Mabhenefiti ema peptide chelates madiki pane glycolic acid uye methionine chelates
| Glycine chelated trace minerals | Methionine chelated trace minerals | Zvicherwa zvidiki zve peptide chelated trace | |
| Fomu rekubatanidza | Mapoka ecarboxyl nemaamino eglycine anogona kusanganiswa nemaion esimbi. | Mapoka emethionine emacarboxyl nemaamino anogona kusanganiswa nemaion esimbi. | Kana yakabatanidzwa nesimbi ions, ine zvakawanda zvekubatana uye hainyatsozadzwa. |
| Basa rezvekudya | Mhando uye mabasa emaamino acids ari ega. | Mhando uye mabasa emaamino acids ari ega. | Iyomhando dzakasiyana-siyanamaamino acids anopa hutano hwakakwana, nepo mapeptides madiki achigona kushanda zvinoenderana. |
| Mhedzisiro yekutora | Glycine chelates inenoMapoka emakabhokisi akasununguka anovapo uye ane simba rinononoka rekunyudzwa. | Methionine chelates inenoMapoka emakabhokisi akasununguka anovapo uye ane simba rinononoka rekunyudzwa. | Ma peptide chelates madiki akaumbwasanganisirakuvapo kwemapoka emakabhokisi akasununguka uye zvine simba rekukurumidza kunyudzwa. |
Chikamu 4 Zita rekutengeserana "Machelate madiki ePeptide-mineral"
MaChelates madiki ePeptide-mineral, sekureva kwezita, ari nyore kuchelate.
Zvinoreva ma peptide ligands madiki, asinganyatsozadzwa nekuda kwehuwandu hwakawanda hwemapoka anobatanidza, Zviri nyore kugadzira multidentate chelate ine zvinhu zvesimbi, ine kugadzikana kwakanaka.
Chikamu 5 Nhanganyaya yeZvigadzirwa zveSmall Peptide-mineral Chelates Series
1. Mhangura diki ine peptide trace mineral chelated copper (zita rekutengeserana: Copper Amino Acid Chelate Feed Grade)
2. Iron ine peptide trace mineral chelated iron (zita rekutengeserana: Ferrous Amino Acid Chelate Feed Grade)
3. Zinc ine peptide trace mineral chelated zinc (zita rekutengeserana: Zinc Amino Acid Chelate Feed Grade)
4. Manganese ine peptide trace mineral chelated manganese (zita rekutengeserana: Manganese Amino Acid Chelate Feed Grade)
Copper Amino Acid Chelate Feed Giredhi
Ferrous Amino Acid Chelate Feed Giredhi
Zinc Amino Acid Chelate Feed Giredhi
Manganese Amino Acid Chelate Feed Giredhi
1. Giredhi reKudya reCopper Amino Acid Chelate
- Zita reChigadzirwa: Copper Amino Acid Chelate Feed Giredhi
- Chitarisiko: Magirafu egirini ebrownish
- Maparamita efizikisikemikari
a) Mhangura: ≥ 10.0%
b) Maamino acid ese: ≥ 20.0%
c) Chiyero cheChelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Mutobvu: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Hunyoro: ≤ 5.0%
h) Kupfava: Zvidimbu zvese zvinopfuura nepamambure makumi maviri, nehukuru hwezvidimbu hukuru hwe60-80 mesh
n=0,1,2,... inoratidza mhangura yakacheneswa ye dipeptides, tripeptides, uye tetrapeptides
Diglycerin
Maumbirwo ema peptide chelates madiki
Hunhu hweCopper Amino Acid Chelate Feed Giredhi
- Chigadzirwa ichi chicherwa cheorganic chelated nemaitiro akakosha ekugadzira mapuroteni ane enzyme yezvirimwa yakachena uye mapuroteni madiki semapeptidi ekugadzira mapuroteni uye zvinhu zvinotevedzana.
- Chigadzirwa ichi chakasimba pamakemikari uye chinogona kuderedza zvakanyanya kukuvara kwacho kuvhitamini nemafuta, nezvimwewo.
- Kushandiswa kwechigadzirwa ichi kunobatsira pakuvandudza kunaka kwechikafu. Chigadzirwa ichi chinonyudzwa kuburikidza nenzira diki dzepeptide neamino acid, zvichideredza makwikwi nekusawirirana nezvimwe zvinhu zvidiki, uye chine mwero wepamusoro wekunyudzwa kwemuviri uye kushandiswa kwacho.
- Mhangura ndiyo inonyanya kukosha mumasero matsvuku eropa, connective tishu, mapfupa, inobatanidzwa mumhando dzakasiyana-siyana dzema enzyme mumuviri, inovandudza mashandiro emuviri, inorwisa mabhakitiriya, inogona kuwedzera uremu hwezuva nezuva, inovandudza muripo wekudya.
Kushandiswa uye Kushanda kweCopper Amino Acid Chelate Feed Grade
| Chinhu chekushandisa | Chiyero chinokurudzirwa (g/t zvinhu zvakakosha) | Zviri mukati me feed yakazara (mg/kg) | Kushanda zvakanaka |
| Dyara | 400～700 | 60～105 | 1. Kuvandudza mashandiro ekubereka uye makore ekushandisa kwembeu dzehove; 2. Wedzera simba revana vari mudumbu nenguruve; 3. Kuvandudza dziviriro yemuviri uye kuramba zvirwere. |
| Nguruve diki | 300～600 | 45～90 | 1. Inobatsira pakuvandudza mashandiro eropa nemasoja emuviri, kuwedzera simba rekudzivirira kushushikana uye simba rekudzivirira zvirwere; 2. Wedzera kukura kwemhuka uye kuvandudza zvakanyanya kushanda zvakanaka kwechikafu. |
| Nguruve dzinokodza | 125 | Ndira 18.5 | |
| Shiri | 125 | Ndira 18.5 | 1. Kuvandudza kushingairira kwemuviri uye kuderedza kufa; 2. Kuvandudza muripo wezvikafu uye kuwedzera mwero wekukura. |
| Mhuka dzemumvura | Hove 40～70 | 6～10.5 | 1. Kurudzira kukura, kuvandudza muripo wezvikafu; 2. Kudzivirira kushushikana, kuderedza kurwara nekufa. |
| Shrimp 150～200 | 22.5～30 | ||
| Mhuka inodya nyama zuva nezuva | Ndira 0.75 | 1. Kudzivirira kushanduka kwemajoini etibial, kusagadzikana kwekufamba kwe "concave back", kuzununguka, kukuvara kwetsandanyama dzemwoyo; 2. Kudzivirira vhudzi kana kuti ganda kuti riome, kuomarara kwebvudzi, kurasikirwa nekukombama kwakajairika, kudzivirira kubuda kwe "mavanga machena" mudenderedzwa reziso; 3. Kudzivirira kudzikira kwehuremu, manyoka, kugadzirwa kwemukaka Kuderera. |
2. Ferrous Amino Acid Chelate Feed Giredhi
- Zita reChigadzirwa: Ferrous Amino Acid Chelate Feed Giredhi
- Chitarisiko: Magirafu egirini ebrownish
- Maparamita efizikisikemikari
a) Simbi: ≥ 10.0%
b) Maamino acid ese: ≥ 19.0%
c) Chiyero cheChelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Mutobvu: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Hunyoro: ≤ 5.0%
h) Kupfava: Zvidimbu zvese zvinopfuura nepamambure makumi maviri, nehukuru hwezvidimbu hukuru hwe60-80 mesh
n=0,1,2,...inoratidza zinc yakacheneswa ye dipeptides, tripeptides, uye tetrapeptides
Hunhu hweFerrous Amino Acid Chelate Feed Grade
- Chigadzirwa ichi imineral inocheneswa nemaitiro echelating akakosha ane enzyme yezvirimwa yakachena, mapuroteni madiki, semacheni echelating uye zvinhu zvinocheneswa;
- Chigadzirwa ichi chinogara kwenguva refu uye chinogona kuderedza zvakanyanya kukuvara kwacho kuvhitamini nemafuta, nezvimwewo. Kushandiswa kwechigadzirwa ichi kunobatsira pakuvandudza kunaka kwechikafu;
- Chigadzirwa ichi chinonyudzwa kuburikidza nenzira diki dzepeptide neamino acid, zvichideredza makwikwi uye kusawirirana nezvimwe zvinhu zvidiki, uye chine mwero wepamusoro wekunyudzwa kwemuviri uye kushandiswa;
- Chigadzirwa ichi chinogona kupfuura nepachivharo cheplacenta negland remazamu, kuita kuti mwana ari mudumbu ave neutano hwakanaka, kuwedzera huremu hwekuzvarwa uye huremu hwekuyamwisa, uye kuderedza huwandu hwekufa; Iron chinhu chakakosha muhemoglobin nemyoglobin, izvo zvinogona kudzivirira zvinobudirira anemia yekushomeka kwesimbi nematambudziko ayo.
Kushandiswa uye Kushanda kweFerrous Amino Acid Chelate Feed Grade
| Chinhu chekushandisa | Chipimo chinokurudzirwa (zvinhu zvakakosha g/t) | Zviri mukati me feed yakazara (mg/kg) | Kushanda zvakanaka |
| Dyara | 300～800 | 45～120 | 1. Kuvandudza mashandiro ekubereka uye nguva yekushandiswa kwembeu dzenguruve; 2. kuvandudza huremu hwekuzvarwa nahwo, huremu hwekuyamwisa uye kufanana kwenguruve kuti ibudirire zvakanaka munguva inotevera; 3. Kuvandudza kuchengetwa kwesimbi munguruve dziri kuyamwa uye huwandu hwesimbi mumukaka kudzivirira kushomeka kwesimbi munguruve dziri kuyamwa. |
| Nguruve diki nenguruve dzinokodza | Nguruve diki 300～600 | 45～90 | 1. Kuvandudza dziviriro yezvirwere zvenguruve, kuwedzera kusimba kwezvirwere uye kuwedzera mwero wekurarama; 2. Wedzera kukura, vandudza kuchinja kwechikafu, wedzera huremu hwemakwayana ekuyamwisa uye kufanana kwawo, uye kuderedza kuwanda kwenguruve dzine chirwere; 3. Kuvandudza huwandu hwemyoglobin nemyoglobin, kudzivirira nekurapa anemia yekushaikwa kwesimbi, kuita kuti ganda renguruve rive dzvuku uye zviri pachena kuti rinovandudza ruvara rwenyama. |
| Nguruve dzinokodza 200～400 | 30～60 | ||
| Shiri | 300～400 | 45～60 | 1. Kuvandudza kuchinja kwechikafu, kuwedzera kukura, kuwedzera kugona kurwisa kushushikana uye kuderedza kufa; 2. Kuvandudza mwero wekukandira mazai, kuderedza mwero wekupwanyika kwezai uye kuwedzera ruvara rwemuzai; 3. Kuvandudza mwero wekuberekesa mazai uye mwero wekubereka mazai pamwe nehuwandu hwekupona kwehuku diki. |
| Mhuka dzemumvura | 200～300 | 30～45 | 1. Kurudzira kukura, kuvandudza kushandurwa kwechikafu; 2. Kuvandudza simba rekudzivirira kushushikana, kuderedza kurwara nekufa. |
3. Zinc Amino Acid Chelate Feed Giredhi
- Zita reChigadzirwa: Zinc Amino Acid Chelate Feed Giredhi
- Chitarisiko: granules dzebrown-yellow
- Maparamita efizikisikemikari
a) Zinc: ≥ 10.0%
b) Maamino acid ese: ≥ 20.5%
c) Chiyero cheChelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Mutobvu: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Hunyoro: ≤ 5.0%
h) Kupfava: Zvidimbu zvese zvinopfuura nepamambure makumi maviri, nehukuru hwezvidimbu hukuru hwe60-80 mesh
n=0,1,2,...inoratidza zinc yakacheneswa ye dipeptides, tripeptides, uye tetrapeptides
Hunhu hweZinc Amino Acid Chelate Feed Giredhi
Chigadzirwa ichi chicherwa cheorganic chelated nemaitiro akakosha echelating ane mapuroteni madiki emakemikari ezvirimwa semapeptidi echelating uye maelement etrace;
Chigadzirwa ichi chakasimba pamakemikari uye chinogona kuderedza zvakanyanya kukuvara kwacho kuvhitamini nemafuta, nezvimwewo.
Kushandiswa kwechigadzirwa ichi kunobatsira pakuvandudza kunaka kwechikafu; Chigadzirwa ichi chinonyudzwa kuburikidza nenzira diki dzepeptide neamino acid, zvichideredza makwikwi nekusawirirana nezvimwe zvinhu zvidiki, uye chine mwero wepamusoro wekunyudzwa kwemuviri uye kushandiswa;
Chigadzirwa ichi chinogona kuvandudza dziviriro yemuviri, kukurudzira kukura, kuwedzera kushanduka kwechikafu uye kuvandudza kupenya kwemvere;
Zinc chinhu chakakosha chine ma enzymes anopfuura 200, epithelial tissue, ribose uye gustatin. Inokurudzira kuwanda nekukurumidza kwemasero ekuravira mumuromo merurimi uye inodzora chido chekudya; inodzivisa mabhakitiriya anokuvadza mudumbu; uye ine basa remishonga inorwisa mabhakitiriya, iyo inogona kuvandudza mashandiro ekugaya uye mashandiro ema enzymes mumatishu nemasero.
Kushandiswa uye Kushanda kweZinc Amino Acid Chelate Feed Grade
| Chinhu chekushandisa | Chipimo chinokurudzirwa (zvinhu zvakakosha g/t) | Zviri mukati me feed yakazara (mg/kg) | Kushanda zvakanaka |
| Mhuka dzesango dzine pamuviri uye dzinoyamwisa | 300～500 | 45～75 | 1. Kuvandudza mashandiro ekubereka uye nguva yekushandiswa kwembeu dzenguruve; 2. Kuvandudza simba remwana ari mudumbu nenguruve, kuwedzera kusimba kwezvirwere, uye kuita kuti dzibudirire pakugadzira zvakanaka padanho rekupedzisira; 3. Kuvandudza mamiriro emuviri wenguruve dzine mimba uye huremu hwekuzvarwa kwenguruve diki. |
| Nguruve inoyamwa, nguruve diki uye nguruve dzinokura dzichikodza | 250～400 | 37.5～60 | 1. Kuvandudza dziviriro yezvirwere zvenguruve, kuderedza manyoka nekufa; 2. Kuvandudza kunaka kwemhuka, kuwedzera kudya kwemhuka, kuwedzera kukura kwayo uye kuvandudza kuchinja kwekudya kwayo; 3. Ita kuti ganda renguruve rive rakajeka uye wedzera kunaka kwemuviri wemhuka uye kunaka kwenyama. |
| Shiri | 300～400 | 45～60 | 1. Kuvandudza kupenya kweminhenga; 2. kuvandudza mwero wekukandira mazai, mwero wekuberekesa mazai uye mwero wekuberekesa mazai, uye kusimbisa kugona kwekupenda kweyolk yezai; 3. Kuvandudza kugona kwekudzivirira kushushikana uye kuderedza kufa; 4. Kuvandudza kushandurwa kwechikafu uye kuwedzera mwero wekukura. |
| Mhuka dzemumvura | Ndira 300 | 45 | 1. Kurudzira kukura, kuvandudza kushandurwa kwechikafu; 2. Kuvandudza simba rekudzivirira kushushikana, kuderedza kurwara nekufa. |
| Mhuka inodya nyama zuva nezuva | 2.4 | 1. Kuvandudza goho remukaka, kudzivirira mastitis nekuora kwemakumbo, uye kuderedza huwandu hwemasero emuviri mumukaka; 2. Kurudzira kukura, kuvandudza kuchinja kwekudya uye kuvandudza mhando yenyama. |
4. Manganese Amino Acid Chelate Feed Giredhi
- Zita reChigadzirwa: Manganese Amino Acid Chelate Feed Giredhi
- Chitarisiko: granules dzebrown-yellow
- Maparamita efizikisikemikari
a) Mn: ≥ 10.0%
b) Maamino acid ese: ≥ 19.5%
c) Chiyero cheChelation: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Mutobvu: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Hunyoro: ≤ 5.0%
h) Kupfava: Zvidimbu zvese zvinopfuura nepamambure makumi maviri, nehukuru hwezvidimbu hukuru hwe60-80 mesh
n=0, 1,2,...inoratidza manganese ane chelated ye dipeptides, tripeptides, uye tetrapeptides
Hunhu hweManganese Amino Acid Chelate Feed Giredhi
Chigadzirwa ichi chicherwa cheorganic chelated nemaitiro akakosha echelating ane mapuroteni madiki emakemikari ezvirimwa semapeptidi echelating uye maelement etrace;
Chigadzirwa ichi chinogara kwenguva refu uye chinogona kuderedza zvakanyanya kukuvara kwacho kuvhitamini nemafuta, nezvimwewo. Kushandiswa kwechigadzirwa ichi kunobatsira pakuvandudza kunaka kwechikafu;
Chigadzirwa ichi chinonyudzwa kuburikidza nenzira diki dzepeptide neamino acid, zvichideredza makwikwi uye kusawirirana nezvimwe zvinhu zvidiki, uye chine mwero wepamusoro wekunyudzwa kwemuviri uye kushandiswa;
Chigadzirwa ichi chinogona kuvandudza kukura kwehuku, kuvandudza kuchinja kwechikafu uye hutano hwayo zvakanyanya; uye kuvandudza kukura kwehuku dzinoisa mazai, kuzvara kwehuku uye kukura kwehuku dzine hutano;
Manganese inodiwa pakukura kwemapfupa uye kuchengetedza mapfupa. Inobatanidzwa zvakanyanya nema enzymes mazhinji; uye inobatanidzwa mukugadzirwa kwema carbohydrate, mafuta nemapuroteni, kubereka uye kupindura kwekudzivirira muviri.
Kushandiswa uye Kushanda kweManganese Amino Acid Chelate Feed Grade
| Chinhu chekushandisa | Chiyero chinokurudzirwa (g/t zvinhu zvakakosha) | Zviri mukati me feed yakazara (mg/kg) | Kushanda zvakanaka |
| Nguruve yekuberekesa | 200～300 | 30～45 | 1. Kukurudzira kukura kwakanaka kwenhengo dzepabonde uye kuvandudza kufamba kwembeu; 2. Kuvandudza kugona kubereka kwenguruve uye kuderedza zvipingamupinyi zvekubereka. |
| Nguruve diki nenguruve dzinokodza | 100～250 | 15～37.5 | 1. Zvinobatsira kuvandudza mashandiro emuviri wekudzivirira chirwere, uye kuvandudza kugona kwekudzivirira kushushikana uye kuramba zvirwere; 2. Kurudzira kukura uye kuvandudza kushandurwa kwechikafu zvakanyanya; 3. Kuvandudza ruvara rwenyama uye mhando yayo, uye kuwedzera huwandu hwenyama isina mafuta akawanda. |
| Shiri | 250～350 | 37.5～52.5 | 1. Kuvandudza kugona kwekudzivirira kushushikana uye kuderedza kufa; 2. Kuvandudza mwero wekukandira mazai, mwero wekuberekesa mazai uye mwero wekuberekesa mazai, kuwedzera kunaka kwegoko remazai uye kuderedza mwero wekutyoka kwemagoko; 3. Kukurudzira kukura kwemapfupa uye kuderedza kuwanda kwezvirwere zvemakumbo. |
| Mhuka dzemumvura | 100～200 | 15～30 | 1. Kukurudzira kukura uye kuvandudza kugona kwayo kurwisa kushushikana uye kuramba zvirwere; 2. Kuvandudza kufamba kwembeu uye huwandu hwemazai akabatanidzwa. |
| Mhuka inodya nyama zuva nezuva | Mombe 1.25 | 1. Kudzivirira kusagadzikana kwekugadzirwa kwemafuta acid uye kukuvara kwemapfupa; 2. Kuvandudza kugona kubereka, kudzivirira kubvisa pamuviri uye kuoma mitezo kwemhuka hadzi mushure mekusununguka, kuderedza kufa kwemhuru nemakwayana, uye kuwedzera huremu hwemhuka diki dzichangoberekwa. | |
| Mbudzi 0.25 |
Chikamu 6 FAB yeChelates diki dzePeptide-mineral
| N/N | F: Hunhu hunoshanda | A: Kusiyana kwemakwikwi | B: Mabhenefiti anokonzerwa nekusiyana kwemakwikwi kune vashandisi |
| 1 | Kudzora kusarudza kwezvinhu zvakagadzirwa | Sarudza hydrolysis ye enzyme yezvirimwa yakachena yema peptides madiki | Kuchengetedzwa kwakanyanya kwezvinhu zvipenyu, kudzivirira kudya vanhu |
| 2 | Tekinoroji yekugaya kugaya kwe enzyme yeprotein mbiri | Huwandu hwakawanda hwemapeptide madiki emamorekuru | Zvimwe "zvinangwa", zvisiri nyore kuzadzisa, zvine basa guru rezvehupenyu uye kugadzikana kuri nani |
| 3 | Tekinoroji yepamusoro yekupfapfaidza nekuomesa | Chigadzirwa chine granular, chine saizi yakaenzana yezvikamu, kuyerera kuri nani, hazvinyore kupinza hunyoro | Ita shuwa kuti zviri nyore kushandisa, kusanganisa kwakafanana mukudya kwakakwana |
| Mvura shoma (≤ 5%), izvo zvinoderedza zvakanyanya simba rinokonzerwa nemavhitamini nema enzymes | Kuvandudza kugadzikana kwezvigadzirwa zvekudya | ||
| 4 | Tekinoroji yepamusoro yekudzora kugadzirwa | Maitiro akakomberedzwa zvachose, mwero wepamusoro wekutonga otomatiki | Hunhu hwakachengeteka uye hwakagadzikana |
| 5 | Tekinoroji yepamusoro yekudzora mhando | Gadzira uye vandudza nzira dzesainzi nedzepamusoro dzekuongorora uye nzira dzekudzora dzekuona zvinhu zvinokanganisa mhando yechigadzirwa, zvakaita seprotein inonyunguduka muacid, kugoverwa kwehuremu hwemamorekuru, maamino acids uye chelating rate | Iva nechokwadi chemhando yepamusoro, simbisa kushanda zvakanaka uye vandudza kushanda zvakanaka |
Chikamu 7 Kuenzanisa Kwemukwikwidzi
Standard VS Standard
Kuenzanisa kwekugoverwa kwepeptide uye mwero wechelation wezvigadzirwa
| Zvigadzirwa zveSustar | Huwandu hwemapeptide madiki (180-500) | Zvigadzirwa zveZinpro | Huwandu hwemapeptide madiki (180-500) |
| AA-Cu | ≥74% | KUWANIKWA-Cu | 78% |
| AA-Fe | ≥48% | KUWANIKWA-FE | 59% |
| AA-Mn | ≥33% | KUWANIKWA-Mn | 53% |
| AA-Zn | ≥37% | KUWANIKA-Zn | 56% |
| Zvigadzirwa zveSustar | Chiyero cheChelation | Zvigadzirwa zveZinpro | Chiyero cheChelation |
| AA-Cu | 94.8% | KUWANIKWA-Cu | 94.8% |
| AA-Fe | 95.3% | KUWANIKWA-FE | 93.5% |
| AA-Mn | 94.6% | KUWANIKWA-Mn | 94.6% |
| AA-Zn | 97.7% | KUWANIKA-Zn | 90.6% |
Chiyero chemapeptide madiki eSustar chakaderera zvishoma pane cheZinpro, uye mwero wechelation wezvigadzirwa zveSustar wakakwira zvishoma pane wezvigadzirwa zveZinpro.
Kuenzanisa kwezviri mukati me17 amino acids muzvigadzirwa zvakasiyana
| Zita re amino acids | Mhangura yaSustar Amino Acid Chelate Giredhi reKudyisa | Zinpro's ZVINOKOSHA mhangura | Sustar's Ferrous Amino Acid C chikafu chemafuta Giredhi | Kuwanikwa kweZinpro simbi | Manganese yeSustar Amino Acid Chelate Giredhi reKudyisa | Kuwanikwa kweZinpro manganese | Zinc yaSustar Asidhi yeAmino Chelate Feed Giredhi | Kuwanikwa kweZinpro zingi |
| asidhi yeaspartic (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| asidhi yeglutamic (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | Ndira 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Maamino acid ese (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Kazhinji, huwandu hwemaamino acids muzvigadzirwa zveSustar hwakakwira kupfuura hwezvigadzirwa zveZinpro.
Chikamu 8 Mhedzisiro yekushandiswa
Mhedzisiro yenzvimbo dzakasiyana dzezvicherwa zvishoma pakugadzirwa uye kunaka kwemazai ehuku dzinokandira mazai panguva yekupedzisira yekukandira mazai.
Maitiro Ekugadzira
- Tekinoroji ye chelation yakanangwa
- Tekinoroji yekubvisa simbi kubva mumafuta ekubikisa
- Tekinoroji yekupfapfaidza nekuomesa nepressure
- Tekinoroji yekuisa mufiriji uye kubvisa hunyoro
- Tekinoroji yepamusoro yekudzora zvakatipoteredza
Appendikisi A: Nzira dzekuongorora kugoverwa kwemamorekuru emapeptides
Kugamuchirwa kwemuyero: GB/T 22492-2008
1 Nheyo yeMuedzo:
Yakaongororwa nekushandisa gel filtration chromatography ine simba guru. Izvi zvinoreva kuti, kushandisa porous filler sechikamu chisingachinji, zvichibva pamusiyano uripo pakati pehukuru hwemamorekuru ezvikamu zvemuenzaniso zvekuparadzanisa, zvakaonekwa pa peptide bond ye ultraviolet absorption wavelength ye 220nm, uchishandisa software yakatsaurirwa yekugadzirisa data yekuona kugoverwa kwemamorekuru ehukama ne gel filtration chromatography (kureva, software yeGPC), ma chromatograms nedata rawo zvakagadziriswa, zvakaverengerwa kuti zviwane saizi yehukuru hwemamorekuru e soya peptide uye huwandu hwekugoverwa.
2. Zvinogadzirisa
Mvura yekuyedza inofanira kusangana nezvinodiwa nemvura yechipiri muGB/T6682, kushandiswa kwema reagents, kunze kwezvimwe zvinhu zvakakosha, kwakachena pakuongorora.
2.1 Zvinoita sema reagents zvinosanganisira acetonitrile (yakachena pa chromatographically), trifluoroacetic acid (yakachena pa chromatographically),
2.2 Zvinhu zvakajairika zvinoshandiswa mu calibration curve yekugoverwa kwemamorekuru: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Zvishandiso nemidziyo
3.1 High Performance Liquid Chromatograph (HPLC): nzvimbo yekushandira kana integrator ine UV detector uye GPC data processing software.
3.2 Chishandiso chekusefa nekubvisa gasi che mobile phase.
3.3 Mari yemagetsi: kukosha kwe 0.000 1g.
Matanho mana ekushanda
4.1 Mamiriro eChromatographic uye kuyedza kugadzirisa masisitimu (mamiriro ekutarisa)
4.1.1 Chinyorwa cheChromatographic: TSKgelG2000swxl300 mm×7.8 mm (dhayamita yemukati) kana mamwe makoramu egel emhando imwe chete ane mashandiro akafanana akakodzera kuongororwa kwemapuroteni nemapeptide.
4.1.2 Danho rekufamba: Acetonitrile + mvura + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Kureba kwehurefu hwechiedza: 220 nm.
4.1.4 Mwero wekuyerera kwemvura: 0.5 mL/min.
4.1.5 Nguva yekuona: maminetsi makumi matatu.
4.1.6 Vhoriyamu yejekiseni yemuenzaniso: 20μL.
4.1.7 Tembiricha yekoramu: tembiricha yemukamuri.
4.1.8 Kuti hurongwa hwe chromatographic husvike pazvinodiwa zvekuongorora, zvakanzi pasi pemamiriro ezvinhu e chromatographic ari pamusoro apa, kushanda zvakanaka kwe gel chromatographic column, kureva, nhamba yemaplates (N), kwaisava pasi pe 10000 yakaverengerwa zvichibva pamatanho e tripeptide standard (Glycine-Glycine-Glycine).
4.2 Kugadzirwa kwemakomba akajairwa ehuwandu hwemamorekuru
Mhinduro dzakasiyana dzepamusoro dze relative molecular mass peptide standard dzine huwandu hwakawanda hwe1 mg / mL dzakagadzirwa ne mobile phase matching, dzakasanganiswa nechikamu chakati, uye dzakazosefetwa kuburikidza ne organic phase membrane ine saizi ye 0.2 μm ~ 0.5 μm ndokupinzwa mu sample, uye ipapo chromatograms ye standards dzakawanikwa. Relative molecular mass calibration curves uye equation dzadzo dzakawanikwa nekunyora logarithm ye relative molecular mass zvichienderana nenguva yekuchengetedza kana ne linear regression.
4.3 Muenzaniso wekurapwa
Yera 10mg yesample nemazvo mu 10mL volumetric flask, wedzera kamobile phase, ultrasonic shaken kwemaminitsi gumi, kuitira kuti sample inyunguduke zvizere uye isanganiswe, yosanganiswa nemobile phase kusvika pachikero, yozoseferwa kuburikidza ne organic phase membrane ine pore size ye 0.2μm~0.5μm, uye filtrate yakaongororwa zvichienderana nemamiriro e chromatographic ari mu A.4.1.
5. Kuverenga kwekugoverwa kwehuwandu hwemamorekuru
Mushure mekuongorora mhinduro yemuenzaniso yakagadzirwa mu4.3 pasi pemamiriro echromatographic e4.1, huwandu hwemamorekuru emuenzaniso uye huwandu hwayo hwekugoverwa hunogona kuwanikwa nekutsiva data rechromatographic remuenzaniso mu calibration curve 4.2 neGPC data processing software. Kugoverwa kwehuwandu hwemamorekuru epeptide dzakasiyana kunogona kuverengerwa nenzira ye peak area normalization, zvichienderana nefomula: X=A/A total×100
Mufomura: X - Chikamu chehuremu hwepeptide ine huwandu hwemamorekuru mupeptide yese mumuenzaniso, %;
A - Nzvimbo yepamusoro yepeptide ine mamorekuru akawanda;
Huwandu A - huwandu hwenzvimbo dzepamusoro dzepeptide yega yega yemorecular mass, yakaverengerwa kusvika panzvimbo imwe chete yedesimali.
6 Kudzokororwa
Musiyano wakakwana pakati pemasarudzo maviri akazvimiririra anowanikwa pasi pemamiriro ekudzokorora haufanirwe kudarika 15% yeavhareji yemasvomhu ezvisarudzo zviviri izvi.
Appendix B: Nzira dzeKuona MaAmino Acids Emahara
Kugamuchirwa kwemuyero: Q/320205 KAVN05-2016
1.2 Zvinogadzirisa zvinhu nezvinhu
Glacial acetic acid: yakachena pakuongorora
Perchloric acid: 0.0500 mol/L
Chiratidzo: 0.1% crystal violet chiratidzo (glacial acetic acid)
2. Kuziva maamino acids emahara
Mienzaniso yacho yakaomeswa pa80°C kweawa imwe chete.
Isa sampuro mumudziyo wakaoma kuti itonhore zviri zvega kusvika patembiricha yemukamuri kana kuti itonhore kusvika patembiricha inoshandisika.
Yera inenge 0.1 g yemuenzaniso (yakarurama kusvika 0.001 g) mubhodhoro re 250 mL rakaoma rakaita sedenderedzwa.
Endai mberi nekukurumidza kunhanho inotevera kuti sampuro isatore hunyoro hwemhepo
Wedzera 25 mL ye glacial acetic acid wobva wasanganisa zvakanaka kweisingasviki maminitsi mashanu.
Wedzera madonhwe maviri echiratidzo che crystal violet
Titrate ne 0.0500 mol / L (±0.001) mhinduro yakajairika ye titration ye perchloric acid kusvika mhinduro yachinja kubva papepuru kuenda pakuguma.
Nyora huwandu hwemushonga wakajairika washandiswa.
Ita bvunzo isina chinhu panguva imwe chete.
3. Kuverenga uye mhedzisiro
Hunhu hweamino acid X huri mu reagent hunoratidzwa sechikamu chehuremu (%) uye hunoverengerwa zvichienderana nefomura: X = C × (V1-V0) × 0.1445/M × 100%, mufomura yetne:
C - Kuwanda kwe perchloric acid solution mumamoles pa litre (mol/L)
V1 - Vhoriyamu inoshandiswa pakuyera sampuli ne perchloric acid solution yakajairika, mu milliliters (mL).
Vo - Vhoriyamu inoshandiswa pakuita kuti titration ive isina chinhu ine mhinduro yakajairwa yeperchloric acid, mu milliliters (mL);
M - Huremu hwemuenzaniso, mumagiramu (g).
0.1445: Avhareji yehuremu hweamino acids yakaenzana ne1.00 mL yemushonga we perchloric acid [c (HClO4) = 1.000 mol / L].
Appendix C: Nzira dzekuongorora huwandu hweSustar's chelation
Kugamuchirwa kwemitemo: Q/70920556 71-2024
1. Nheyo yekusarudza (Fe semuenzaniso)
MaAmino acid iron complexes ane kunyungudika kwakaderera muanhydrous ethanol uye maFree metal ions anonyungudika muanhydrous ethanol, musiyano wekunyungudika pakati pezviviri muanhydrous ethanol wakashandiswa kuona chelation rate yeamino acid iron complexes.
2. Zvinogadzirisa & Mhinduro
Anhydrous ethanol; zvimwe zvakafanana nechikamu 4.5.2 muGB/T 27983-2011.
3. Matanho ekuongorora
Ita miedzo miviri panguva imwe chete. Yera 0.1g yemuenzaniso wakaomeswa pa103±2℃ kweawa imwe chete, zvichienderana ne0.0001g, wedzera 100mL yeanhydrous ethanol kuti inyungudike, firita, firita zvasara zvakashambidzwa ne100mL yeanhydrous ethanol kwekanenge katatu, wobva waisa zvasara mu 250mL conical flask, wedzera 10mL yesulfuric acid solution zvichienderana nechikamu 4.5.3 muGB/T27983-2011, wobva waita matanho anotevera zvichienderana nechikamu 4.5.3 chekuti “Pisa kuti unyungudike wozorega zvichitonhorera” muGB/T27983-2011. Ita bvunzo isina chinhu panguva imwe chete.
4. Kuziva huwandu hwesimbi
4.1 Nheyo yekugadza yakafanana nechikamu 4.4.1 muGB/T 21996-2008.
4.2. Zvinogadzirisa uye Mhinduro
4.2.1 Asidhi yakasanganiswa: Wedzera 150mL yesulfuric acid uye 150mL yephosphoric acid ku700mL yemvura wosanganisa zvakanaka.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, yakagadzirwa zvichienderana neGB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, yakagadzirwa zvichienderana neGB/T601.
4.3 Matanho ekuongorora
Ita miedzo miviri panguva imwe chete. Yera 0.1g yemuenzaniso, yakakodzera 020001g, isa mu 250mL conical flask, wedzera 10mL ye mixed acid, mushure mekunyungudika, wedzera 30ml yemvura nemadonhwe mana e sodium dianiline sulfonate indicator solution, wobva waita matanho anotevera zvichienderana nechikamu 4.4.2 mu GB/T21996-2008. Ita bvunzo isina chinhu panguva imwe chete.
4.4 Kumiririrwa kwemigumisiro
Huwandu hwesimbi X1 hweamino acid iron complexes maererano nechikamu chesimbi, kukosha kwakaratidzwa mu%, kwakaverengerwa zvichienderana nefomula (1):
X1=(V-V0)×C×M×10-3×100
Mufomura: V - vhoriyamu ye cerium sulfate yakajairwa inonwiwa kuti mhinduro yekuedza iwedzere, mL;
V0 - cerium sulfate yakajairika inonwiwa kuti mhinduro isina chinhu iwane titration, mL;
C - Kuwanda chaiko kwe cerium sulfate solution, mol/L
5. Kuverengwa kwesimbi iri mu chelates
Huremu hwesimbi X2 muchelate maererano nechikamu chesimbi, kukosha kwacho kwakataurwa mu %, kwakaverengerwa zvichienderana nefomura: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
Mufomura: V1 - vhoriyamu ye cerium sulfate yakajairwa inonwiwa kuti mhinduro yekuedza iwedzere, mL;
V2 - cerium sulfate yakajairika inonwiwa kuti mhinduro isina chinhu iwane titration, mL;
C - Kuwanda chaiko kwe cerium sulfate solution, mol/L;
0.05585 - huremu hwesimbi yesimbi inoburitswa mumagiramu akaenzana ne1.00 mL ye cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass yemuenzaniso, g. Tora avhareji yemasvomhu yemhedzisiro yesarudzo yakafanana semhedzisiro yesarudzo, uye mutsauko wakakwana wemhedzisiro yesarudzo yakafanana haupfuuri 0.3%.
6. Kuverengwa kwehuwandu hwe chelation
Chiyero cheChelation X3, kukosha kwakaratidzwa mu %, X3 = X2/X1 × 100
Appendix C: Nzira dzekuongorora huwandu hweZinpro's chelation
Kugamuchirwa kwemuyero: Q/320205 KAVNO7-2016
1. Zvinogadzirisa zvinhu uye zvinhu
a) Glacial acetic acid: yakachena pakuongorora; b) Perchloric acid: 0.0500mol/L; c) Chiratidzo: 0.1% crystal violet indicator (glacial acetic acid)
2. Kuziva maamino acids emahara
2.1 Mienzaniso yacho yakaomeswa pa80°C kweawa imwe chete.
2.2 Isa sampuro mumudziyo wakaoma kuti itonhore zviri zvega kusvika patembiricha yemukamuri kana kuti itonhore kusvika patembiricha inoshandisika.
2.3 Yera inenge 0.1 g yemuenzaniso (yakarurama kusvika 0.001 g) mubhodhoro re 250 mL rakaoma rakaita sedenderedzwa.
2.4 Endai mberi nekukurumidza kunhanho inotevera kuti sample isatore hunyoro hwemhepo.
2.5 Wedzera 25mL ye glacial acetic acid wosanganisa zvakanaka kweisingasviki 5min.
2.6 Wedzera madonhwe maviri echiratidzo che crystal violet.
2.7 Titrate ne 0.0500mol/L (±0.001) mhinduro yakajairika ye titration ye perchloric acid kusvika mhinduro yachinja kubva papepuru kuenda pagirini kwemasekonzi gumi nemashanu pasina kuchinja ruvara senzvimbo yekupedzisira.
2.8 Nyora huwandu hwemushonga wakajairwa washandiswa.
2.9 Ita bvunzo isina chinhu panguva imwe chete.
3. Kuverenga uye mhedzisiro
Hunhu hweamino acid X huri mu reagent hunoratidzwa sechikamu chehuremu (%), zvichiverengerwa zvichienderana nefomura (1): X=C×(V1-V0) ×0.1445/M×100%........(1)
Mufomura: C - kuwanda kwemushonga we perchloric acid wakajairwa mumamoles pa litre (mol/L)
V1 - Vhoriyamu inoshandiswa pakuyera sampuli ne perchloric acid solution yakajairika, mu milliliters (mL).
Vo - Vhoriyamu inoshandiswa pakuita kuti titration ive isina chinhu ine mhinduro yakajairwa yeperchloric acid, mu milliliters (mL);
M - Huremu hwemuenzaniso, mumagiramu (g).
0.1445 - Avhareji yehuremu hweamino acids yakaenzana ne1.00 mL yemushonga we perchloric acid [c (HClO4) = 1.000 mol / L].
4. Kuverengwa kwehuwandu hwe chelation
Mwero we chelation wemuenzaniso unoratidzwa sechikamu chehuremu (%), zvichiverengerwa zvichienderana nefomura (2): mwero we chelation = (huwandu hweamino acid - huwandu hweamino acid husina maamino acid)/huwandu hweamino acid × 100%.
Nguva yekutumira: Gunyana-17-2025
