2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Zvinhu zvakajairika zvinoshandiswa mu calibration curve yekugoverwa kwemamorekuru: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Zvishandiso nemidziyo
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Kazhinji, huwandu hwemaamino acids muzvigadzirwa zveSustar hwakakwira kupfuura hwezvigadzirwa zveZinpro.
Chikamu 8 Mhedzisiro yekushandiswa
Mhedzisiro yenzvimbo dzakasiyana dzezvicherwa zvishoma pakugadzirwa uye kunaka kwemazai ehuku dzinokandira mazai panguva yekupedzisira yekukandira mazai.
Maitiro Ekugadzira
Tekinoroji ye chelation yakanangwa
Tekinoroji yekubvisa simbi kubva mumafuta ekubikisa
Tekinoroji yekupfapfaidza nekuomesa nepressure
Tekinoroji yekuisa mufiriji uye kubvisa hunyoro
Tekinoroji yepamusoro yekudzora zvakatipoteredza
Appendikisi A: Nzira dzekuongorora kugoverwa kwemamorekuru emapeptides
Kugamuchirwa kwemuyero: GB/T 22492-2008
1 Nheyo yeMuedzo:
Yakaongororwa nekushandisa gel filtration chromatography ine simba guru. Izvi zvinoreva kuti, kushandisa porous filler sechikamu chisingachinji, zvichibva pamusiyano uripo pakati pehukuru hwemamorekuru ezvikamu zvemuenzaniso zvekuparadzanisa, zvakaonekwa pa peptide bond ye ultraviolet absorption wavelength ye 220nm, uchishandisa software yakatsaurirwa yekugadzirisa data yekuona kugoverwa kwemamorekuru ehukama ne gel filtration chromatography (kureva, software yeGPC), ma chromatograms nedata rawo zvakagadziriswa, zvakaverengerwa kuti zviwane saizi yehukuru hwemamorekuru e soya peptide uye huwandu hwekugoverwa.
2. Zvinogadzirisa
Mvura yekuyedza inofanira kusangana nezvinodiwa nemvura yechipiri muGB/T6682, kushandiswa kwema reagents, kunze kwezvimwe zvinhu zvakakosha, kwakachena pakuongorora.
2.1 Zvinoita sema reagents zvinosanganisira acetonitrile (yakachena pa chromatographically), trifluoroacetic acid (yakachena pa chromatographically),
2.2 Zvinhu zvakajairika zvinoshandiswa mu calibration curve yekugoverwa kwemamorekuru: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Zvishandiso nemidziyo
3.1 High Performance Liquid Chromatograph (HPLC): nzvimbo yekushandira kana integrator ine UV detector uye GPC data processing software.
3.2 Chishandiso chekusefa nekubvisa gasi che mobile phase.
3.3 Mari yemagetsi: kukosha kwe 0.000 1g.
Matanho mana ekushanda
4.1 Mamiriro eChromatographic uye kuyedza kugadzirisa masisitimu (mamiriro ekutarisa)
- 4.1.1 Chinyorwa cheChromatographic: TSKgelG2000swxl300 mm×7.8 mm (dhayamita yemukati) kana mamwe makoramu egel emhando imwe chete ane mashandiro akafanana akakodzera kuongororwa kwemapuroteni nemapeptide.
- 4.1.2 Danho rekufamba: Acetonitrile + mvura + trifluoroacetic acid = 20 + 80 + 0.1.
- 4.1.3 Kureba kwehurefu hwechiedza: 220 nm.
- 4.1.4 Mwero wekuyerera kwemvura: 0.5 mL/min.
- 4.1.5 Nguva yekuona: maminetsi makumi matatu.
- 4.1.6 Vhoriyamu yejekiseni yemuenzaniso: 20μL.
- 4.1.7 Tembiricha yekoramu: tembiricha yemukamuri.
- 4.1.8 Kuti hurongwa hwe chromatographic husvike pazvinodiwa zvekuongorora, zvakanzi pasi pemamiriro ezvinhu e chromatographic ari pamusoro apa, kushanda zvakanaka kwe gel chromatographic column, kureva, nhamba yemaplates (N), kwaisava pasi pe10000 yakaverengerwa zvichibva pamatanho e tripeptide standard (Glycine-Glycine-Glycine).
- 4.2 Kugadzirwa kwemakomba akajairwa ehuwandu hwemamorekuru
- Mhinduro dzakasiyana dzepamusoro dze relative molecular mass peptide standard dzine huwandu hwakawanda hwe1 mg / mL dzakagadzirwa ne mobile phase matching, dzakasanganiswa nechikamu chakati, uye dzakazosefetwa kuburikidza ne organic phase membrane ine saizi ye 0.2 μm ~ 0.5 μm ndokupinzwa mu sample, uye ipapo chromatograms ye standards dzakawanikwa. Relative molecular mass calibration curves uye equation dzadzo dzakawanikwa nekunyora logarithm ye relative molecular mass zvichienderana nenguva yekuchengetedza kana ne linear regression.
4.3 Muenzaniso wekurapwa
Yera 10mg yesample nemazvo mu 10mL volumetric flask, wedzera kamobile phase, ultrasonic shaken kwemaminitsi gumi, kuitira kuti sample inyunguduke zvizere uye isanganiswe, yosanganiswa nemobile phase kusvika pachikero, yozoseferwa kuburikidza ne organic phase membrane ine pore size ye 0.2μm ~ 0.5μm, uye filtrate yakaongororwa zvichienderana nemamiriro e chromatographic ari mu A.4.1.
- 5. Kuverenga kwekugoverwa kwehuwandu hwemamorekuru
- Mushure mekuongorora mhinduro yemuenzaniso yakagadzirwa mu4.3 pasi pemamiriro echromatographic e4.1, huwandu hwemamorekuru emuenzaniso uye huwandu hwayo hwekugoverwa hunogona kuwanikwa nekutsiva data rechromatographic remuenzaniso mu calibration curve 4.2 neGPC data processing software. Kugoverwa kwehuwandu hwemamorekuru epeptide dzakasiyana kunogona kuverengerwa nenzira ye peak area normalization, zvichienderana nefomula: X=A/A total×100
- Mufomura: X - Chikamu chehuremu hwepeptide ine huwandu hwemorekuru mupeptide yese mumuenzaniso, %;
- A - Nzvimbo yepamusoro yepeptide ine mamorekuru akawanda;
- Huwandu A - huwandu hwenzvimbo dzepamusoro dzepeptide yega yega yemorecular mass, yakaverengerwa kusvika panzvimbo imwe chete yedesimali.
- 6 Kudzokororwa
- Musiyano wakakwana pakati pemasarudzo maviri akazvimiririra anowanikwa pasi pemamiriro ekudzokorora haufanirwe kudarika 15% yeavhareji yemasvomhu ezvisarudzo zviviri izvi.
- Appendix B: Nzira dzeKuona MaAmino Acids Emahara
- Kugamuchirwa kwemuyero: Q/320205 KAVN05-2016
- 1.2 Zvinogadzirisa zvinhu nezvinhu
- Glacial acetic acid: yakachena pakuongorora
- Perchloric acid: 0.0500 mol/L
- Chiratidzo: 0.1% crystal violet chiratidzo (glacial acetic acid)
- 2. Kuziva maamino acids emahara
Mienzaniso yacho yakaomeswa pa80°C kweawa imwe chete.
Isa sampuro mumudziyo wakaoma kuti itonhore zviri zvega kusvika patembiricha yemumba kana kuti itonhore kusvika patembiricha inoshandisika.Yera inenge 0.1 g yemuenzaniso (yakarurama kusvika 0.001 g) mubhodhoro re 250 mL rakaoma rakaita sedenderedzwa.Endai mberi nekukurumidza kunhanho inotevera kuti sampuro isatore hunyoro hwemhepoWedzera 25 mL ye glacial acetic acid wobva wasanganisa zvakanaka kweisingasviki maminitsi mashanu.Wedzera madonhwe maviri echiratidzo che crystal violetTitrate ne 0.0500 mol / L (±0.001) mhinduro yakajairika ye titration ye perchloric acid kusvika mhinduro yachinja kubva papepuru kuenda pakuguma.
Nyora huwandu hwemushonga wakajairika washandiswa.
- Ita bvunzo isina chinhu panguva imwe chete.
- 3. Kuverenga uye mhedzisiro
- Hunhu hweamino acid X huri mu reagent hunoratidzwa sechikamu chehuremu (%) uye hunoverengerwa zvichienderana nefomura: X = C × (V1-V0) × 0.1445/M × 100%, mufomura yetne:
- C - Kuwanda kwe perchloric acid solution mumamoles pa litre (mol/L)
- V1 - Vhoriyamu inoshandiswa pakuyera sampuli ne perchloric acid solution yakajairika, mu milliliters (mL).
- Vo - Vhoriyamu inoshandiswa pakuita kuti titration ive isina chinhu ine mhinduro yakajairwa yeperchloric acid, mu milliliters (mL);
M - Huremu hwemuenzaniso, mumagiramu (g).
| 0.1445: Avhareji yehuremu hweamino acids yakaenzana ne1.00 mL yemushonga we perchloric acid [c (HClO4) = 1.000 mol / L]. | 4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, yakagadzirwa zvichienderana neGB/T601. | |
| Kugamuchirwa kwemitemo: Q/70920556 71-2024 | 1. Nheyo yekusarudza (Fe semuenzaniso) | MaAmino acid iron complexes ane kunyungudika kwakaderera muanhydrous ethanol uye maFree metal ions anonyungudika muanhydrous ethanol, musiyano wekunyungudika pakati pezviviri muanhydrous ethanol wakashandiswa kuona chelation rate yeamino acid iron complexes. |
| Mufomura: V1 - vhoriyamu ye cerium sulfate yakajairwa inonwiwa kuti mhinduro yekuedza iwedzere, mL; | Anhydrous ethanol; zvimwe zvakafanana nechikamu 4.5.2 muGB/T 27983-2011. | 3. Matanho ekuongorora |
| Ita miedzo miviri panguva imwe chete. Yera 0.1g yemuenzaniso wakaomeswa pa103±2℃ kweawa imwe chete, zvichienderana ne0.0001g, wedzera 100mL yeanhydrous ethanol kuti inyungudike, firita, firita zvasara zvakashambidzwa ne100mL yeanhydrous ethanol kwekanenge katatu, wobva waisa zvasara mu 250mL conical flask, wedzera 10mL yesulfuric acid solution zvichienderana nechikamu 4.5.3 muGB/T27983-2011, wobva waita matanho anotevera zvichienderana nechikamu 4.5.3 chekuti “Pisa kuti unyungudike wozorega zvichitonhorera” muGB/T27983-2011. Ita bvunzo isina chinhu panguva imwe chete. | 4. Kuziva huwandu hwesimbi | 4.1 Nheyo yekugadza yakafanana nechikamu 4.4.1 muGB/T 21996-2008. |
4.2. Zvinogadzirisa uye Mhinduro
| 4.2.1 Asidhi yakasanganiswa: Wedzera 150mL yesulfuric acid uye 150mL yephosphoric acid ku700mL yemvura wosanganisa zvakanaka. | 4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, yakagadzirwa zvichienderana neGB/T603. | 4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, yakagadzirwa zvichienderana neGB/T601. | |
| 4.3 Matanho ekuongorora | Ita miedzo miviri panguva imwe chete. Yera 0.1g yemuenzaniso, yakakodzera 020001g, isa mu 250mL conical flask, wedzera 10mL ye mixed acid, mushure mekunyungudika, wedzera 30ml yemvura nemadonhwe mana e sodium dianiline sulfonate indicator solution, wobva waita matanho anotevera zvichienderana nechikamu 4.4.2 mu GB/T21996-2008. Ita bvunzo isina chinhu panguva imwe chete. | 4.4 Kumiririrwa kwemigumisiro | Huwandu hwese hwesimbi X1 hweamino acid iron complexes maererano nechikamu chesimbi, kukosha kwakaratidzwa mu%, kwakaverengerwa zvichienderana nefomula (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - cerium sulfate yakajairika inonwiwa kuti mhinduro isina chinhu iwane titration, mL; | V0 - cerium sulfate yakajairika inonwiwa kuti mhinduro isina chinhu iwane titration, mL; | C - Kuwanda chaiko kwe cerium sulfate solution, mol/L5. Kuverengwa kwesimbi iri mu chelatesHuremu hwesimbi X2 muchelate maererano nechikamu chesimbi, kukosha kwacho kwakataurwa mu %, kwakaverengerwa zvichienderana nefomura: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Mufomura: V1 - vhoriyamu ye cerium sulfate yakajairwa inonwiwa kuti mhinduro yekuedza iwedzere, mL; | V2 - cerium sulfate yakajairika inonwiwa kuti mhinduro isina chinhu iwane titration, mL;nom1-Mass yemuenzaniso, g. Tora avhareji yemasvomhu yemhedzisiro yesarudzo yakafanana semhedzisiro yesarudzo, uye mutsauko wakakwana wemhedzisiro yesarudzo yakafanana haupfuuri 0.3%. | 0.05585 - huremu hwesimbi yesimbi inoburitswa mumagiramu akaenzana ne1.00 mL ye cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Mass yemuenzaniso, g. Tora avhareji yemasvomhu yemhedzisiro yesarudzo yakafanana semhedzisiro yesarudzo, uye mutsauko wakakwana wemhedzisiro yesarudzo yakafanana haupfuuri 0.3%. | 6. Kuverengwa kwehuwandu hwe chelationChiyero cheChelation X3, kukosha kwakaratidzwa mu %, X3 = X2/X1 × 100Appendix C: Nzira dzekuongorora huwandu hweZinpro's chelation |
Kugamuchirwa kwemuyero: Q/320205 KAVNO7-2016
1. Zvinogadzirisa zvinhu uye zvinhu
a) Glacial acetic acid: yakachena pakuongorora; b) Perchloric acid: 0.0500mol/L; c) Chiratidzo: 0.1% crystal violet indicator (glacial acetic acid)
2. Kuziva maamino acids emahara
2.1 Mienzaniso yacho yakaomeswa pa80°C kweawa imwe chete.
2.2 Isa sampuro mumudziyo wakaoma kuti itonhore zviri zvega kusvika patembiricha yemukamuri kana kuti itonhore kusvika patembiricha inoshandisika.
2.3 Yera inenge 0.1 g yemuenzaniso (yakarurama kusvika 0.001 g) mubhodhoro re 250 mL rakaoma rakaita sedenderedzwa.
2.4 Endai mberi nekukurumidza kunhanho inotevera kuti sample isatore hunyoro hwemhepo.
2.5 Wedzera 25mL ye glacial acetic acid wosanganisa zvakanaka kweisingasviki 5min.
2.6 Wedzera madonhwe maviri echiratidzo che crystal violet.
2.7 Titrate ne 0.0500mol/L (±0.001) mhinduro yakajairika ye titration ye perchloric acid kusvika mhinduro yachinja kubva papepuru kuenda pagirini kwemasekonzi gumi nemashanu pasina kuchinja ruvara senzvimbo yekupedzisira.
2.8 Nyora huwandu hwemushonga wakajairwa washandiswa.
2.9 Ita bvunzo isina chinhu panguva imwe chete.
- 3. Kuverenga uye mhedzisiro
- Katarani
- Physicochemical parameters
V1 - Vhoriyamu inoshandiswa pakuyera sampuli ne perchloric acid solution yakajairika, mu milliliters (mL).
Vo - Vhoriyamu inoshandiswa pakuita kuti titration ive isina chinhu ine mhinduro yakajairwa yeperchloric acid, mu milliliters (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Kero: Nhamba 147 Qingpu Road, Shouan Town, Pujiang County, chengdu City, Sichuan Province, China
Nhare: 86-18880477902
Zvigadzirwa
Zvicherwa zvisingawanikwe
- Zvicherwa zveOrganic
- ChiSwahili
- Basa rakagadzirirwa
- Zvinongedzo zvinokurumidza
Mbiri Yekambani
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| ChiGujarati | Dzvanya kuti ubvunze | © Kodzero Dzese Dzakachengetedzwa - 2010-2025. | Mepu yenzvimbo Tsvaga yepamusoro Runhare |
| Nhare | 86-18880477902 | ChiJavanese | E-mail |
| 8618880477902 | ChiChinese | ChiFrench | |
| Bird | ChiChinese | ChiFrench | ChiJerimani ChiSpanish |
| Aquatic animals | ChiJapanese | ChiKorean | ChiArabic ChiGiriki |
| ChiTurkey | ChiItalian | ||
| Ruminant animal g/head day | January 0.75 | ChiIndonesian ChiAfrikaans ChiSwedish |
ChiPolish
- ChiBasque
- Katarani
- Physicochemical parameters
ChiHindi
Layo
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
ChiBulgarian
- ChiCebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- ChiCroatian
ChiDutch
| Application object | ChiUrdu ChiVietnamese | Content in full-value feed (mg/kg) | Efficacy |
| ChiGujarati | chiHaiti | ChiHausa | Kinyarwanda ChiHmong ChiHungarian |
| Piglets and fattening pigs | ChiIgbo | ChiJavanese | ChiKannada ChiKhmer ChiKedhi |
| Kiyagizi | Ratini | ||
| Bird | 300～400 | 45～60 | ChiMacedonian ChiMalay ChiMalayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
ChiNorwegian
- Pashito
- Appearance: brownish-yellow granules
- Physicochemical parameters
ChiSebhiya
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
ChiSindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
ChiSwahili
Tajik
ChiTamil
ChiTelugu
ChiThai
| Application object | ChiUrdu ChiVietnamese | Content in full-value feed (mg/kg) | Efficacy |
| ChiYiddish | ChiYoruba | ChiZulu | Kinyarwanda ChiOrya ChiTeki |
| Ugwa | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
